Progress in Allergy Signal Research on Mast Cells:Regulation of Allergic Airway Inflammation Through Toll-Like Receptor 4–Mediated Modification of Mast Cell Function

Journal of Pharmacological Sciences

Toshinori Nakayama¹  Masakatsu Yamashita¹ 

[1] Department of Immunology, Graduate School of Medicine, Chiba University, Japan

关键词: Toll-like receptor 4 (TLR4);    mast cell;    Th2 cytokine;    NF-κB;    GATA1;    allergy;   

DOI  :  10.1254/jphs.FM0070202

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(29)Cited-By(12)In a mouse experimental asthma model, the administration of bacterial lipopolysaccharide (LPS), particularly at low doses, enhances the levels of ovalbumin (OVA)-induced eosinophilic airway inflammation. In an effort to clarify the cellular and molecular basis for the LPS effect, we demonstrate that the OVA-induced eosinophilic inflammation in the lung is dramatically increased by administration of LPS at the priming phase in wild-type mice, whereas such an increase was not observed in mast cell deficient mice. Adoptive transfer of bone marrow-derived mast cells (BMMC) from wild type but not from Toll-like receptor 4 (TLR4)-deficient mice restored the increased eosinophilic inflammation in mast cell–deficient mice. Moreover, in vitro analysis revealed that treatment of BMMC with LPS resulted in sustained up-regulation of GATA1 expression and increased production of Th2 cytokines (IL-4, IL-5, and IL-13) upon restimulation. Thus, mast cells appear to control allergic airway inflammation after their activation and modulation through TLR4-mediated induction of GATA1 proteins and subsequent increase in Th2 cytokine production.

【 授权许可】

Unknown