Th2 Immune Responses and Alternatively Activated Macrophages (AAMacs) in Helminth Infection in Aged Mice

Journal of Veterinary Medical Science

Yasuhiro SUGAWARA¹  Noritsugu AZUMA¹  Yuichi TSUNOKA¹  Motoko MORIMOTO¹  Sachi ONODERA¹ 

[1] School of Food, Agricultural and Environmental Sciences, Miyagi University

关键词: aging;    macrophages;    nematode;    small intestine;    Th2 cytokine;   

DOI  :  10.1292/jvms.10-0269

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(27)Cited-By(10)This study aims to understand Th2 immune responses and alternative macrophage activation against nematode parasites in aged mice. Eighteen-month (18 M) and three-month (3 M) old C3H/HeN mice were inoculated with Heligmosomoides polygyrus (Hp) larvae. Real-time PCR analysis indicated that interleukin (IL)-4 and IL-13 gene expression was elevated in both groups after infection, but the expression level was significantly low in 18 M mice. Macrophage phenotype was monitored by measuring arginase-1 gene expression and immunofluorescence staining in small intestine, showing a decrease in the number of alternatively activated macrophages (AAMacs) around worm cysts in 18 M mice. These results suggest that the Th2 immune response in aged mice against a nematode parasite was not sufficiently induced to promote AAMacs.

【 授权许可】

Unknown