Douchi (fermented Glycine max Merr.) alleviates atopic dermatitis-like skin lesions in NC/Nga mice by regulation of PKC and IL-4

BMC Complementary and Alternative Medicine

Research Article

In-Sik Park¹  Sang-hyun Ahn²  A-Ram Jung³  Jin-Hong Cheon⁴  Kibong Kim⁴  Sun-Young Park⁵  Seung-Il Jeong⁶ 

[1] Department of Anatomy, College of Korean Medicine, Dongguk University, 123 Dongdae-ro, 38066, Gyeongju, Gyeongsangbuk-do, Republic of Korea;Department of Anatomy, College of Korean Medicine, Semyung University, 65 Semyung-ro, 27136, Jecheon-si, Chungcheongbuk-do, Republic of Korea;Department of Korean Pediatrics, Hospital of Korean Medicine, Pusan National University, 20 Geumo-ro, Mulgeum-eup, 50612, Yangsan-si, Gyeongsangnam-do, Republic of Korea;Department of Korean Pediatrics, Hospital of Korean Medicine, Pusan National University, 20 Geumo-ro, Mulgeum-eup, 50612, Yangsan-si, Gyeongsangnam-do, Republic of Korea;Department of Korean Pediatrics, School of Korean Medicine, Pusan National University, 49 Pusandaehak-ro, Mulgeum-eup, 50612, Yangsan-si, Gyeongsangnam-do, Republic of Korea;Department of Physiology, College of Korean Medicine, Semyung University, 65 Semyung-ro, 27136, Jecheon-si, Chungcheongbuk-do, Republic of Korea;Jeonju AgroBio-Materials Institute, 54810, Jeonju, Jeollabuk-do, Republic of Korea;

关键词: Douchi;    Fermented soybean;    Atopic dermatitis;    Th2 cytokine;    PKC;   

DOI  :  10.1186/s12906-016-1394-4

 received in 2016-02-12, accepted in 2016-10-11,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundDouchi (fermented Glycine max Merr.) is produced from fermented soybeans, which is widely used in traditional herbal medicine. In this study, we investigated whether Douchi attenuates protein kinase C (PKC) and interleukin (IL)-4 response and cutaneous inflammation in Atopic dermatitis (AD)-like NC/Nga mice.MethodsTo induce AD-like skin lesions, D. farinae antigen was applied to the dorsal skin of 3-week-old NC/Nga mice. After inducing AD, Douchi extract was administered 20 mg/kg daily for 3 weeks to the Douchi-treated mice group. We identified the changes of skin barrier and Th2 differentiation through PKC and IL-4 by immunohistochemistry.ResultsDouchi treatment of NC/Nga mice significantly reduced clinical scores (p < 0.01) and histological features. The levels of PKC and IL-4 were significantly reduced in the Douchi-treated group (p < 0.01). The reduction of IL-4 and PKC led to decrease of inflammatory factors such as substance P, inducible nitric oxide synthase (iNOS) and Matrix metallopeptidase 9 (MMP-9) (all p < 0.01). Douchi also down-regulated Th1 markers (IL-12, TNF-α) as well as Th2 markers (IL-4, p-IκB) (p < 0.01).ConclusionDouchi alleviates AD-like skin lesions through suppressing of PKC and IL-4. These results also lead to diminish levels of substance P, iNOS and MMP-9 in skin lesions. Therefore, Douchi may have potential applications for the prevention and treatment of AD.

【 授权许可】

CC BY   
© The Author(s). 2016