Anti-Obesity Effects of Selective Agonists to the β3-Adrenergic Receptor in Dogs. I. The Presence of Canine β3-Adrenergic Receptor and in vivo Lipomobilization by Its Agonists

Journal of Veterinary Medical Science

Eiji UCHIDA³  Masayuki SAITO¹  Toshihide YOSHIDA²  Noriyasu SASAKI¹  Masayoshi NIIYAMA³ 

[1] Laboratory of Biochemistry, Department of Biomedical Sciences, School of Veterinary Medicine, Hokkaido University;Department of Internal Medicine, Kyoto Prefectural University of Medicine;Department of Internal Medicine II, Rakuno Gakuen University

关键词: adipose tissue;    adrenergic agonist;    β3-adrenergic receptor;    canine;    lipomobilization;   

DOI  :  10.1292/jvms.60.459

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(5)It is known that in rodents and humans the β3-adrenergic receptor (β3-AR) is present primarily in adipocytes and plays a significant role in the adrenergic stimulation of lipolysis. We examined the expression of β3-AR mRNA in the dog and the lipomobilizing effects of β3-AR-selective agonists in vivo. Reverse transcription polymerase chain reaction of RNA extracted from dog adipose tissue produced a cDNA fragment, the nucleotide sequence of which was highly homologous to the corresponding regions of human (86.4%) and mouse (79.5%) β3-AR cDNA. The β3-AR mRNA was present at high levels in subcutaneous and visceral adipose tissues, but undetectable in other organs. When a selective β3-AR agonist, CL316, 243, was infused intravenously into beagle dogs, the plasma level of free fatty acid increased in 30 min and persisted at higher levels for several hours. ICI D7114, another β3-AR agonist, also showed a similar lipomobilizing effect, but with lower potency. β3-AR agonist infusion also increased the plasma insulin level. These results suggested that functional β3-AR is present in adipose tissues of the dog and that it is effective for in vivo lipomobilization.

【 授权许可】

Unknown