Anti-Obesity Effects of Selective Agonists to the β3-Adrenergic Receptor in Dogs. II. Recruitment of Thermogenic Brown Adipocytes and Reduction of Adiposity after Chronic Treatment with a β3-Adrenergic Agonist

Journal of Veterinary Medical Science

Eiji UCHIDA³  Masayuki SAITO¹  Toshihide YOSHIDA²  Noriyasu SASAKI¹  Masayoshi NIIYAMA³ 

[1] Laboratory of Biochemistry, Department of Biomedical Sciences, School of Veterinary Medicine, Hokkaido University;Department of Internal Medicine, Kyoto Prefectural University of Medicine;Department of Internal Medicine II, Rakuno Gakuen University

关键词: adipose tissue;    β3-adrenergic receptor;    canine;    obesity;    uncoupling protein;   

DOI  :  10.1292/jvms.60.465

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Cited-By(14)The aim of this study was to evaluate the effectiveness of β3-adrenergic agonists for the treatment and prevention of obesity in the dog. When a selective β3-adrenergic agonist, CL316, 243 (0.1 mg/kg), was given orally to adult beagles every day for 5-7 weeks, body weight and girth were decreased compared with control placebo-treated dogs. Gross anatomical examinations revealed no noticeable abnormalities in CL316, 243-treated dogs, except an apparent decrease in abdominal fat. Immunohistochemical examination of perirenal adipose tissue showed a remarkable increase in brown adipocytes expressing a thermogenic protein, uncoupling protein (UCP). The increased expression of UCP and its mRNA in CL316, 243-treated dogs was also confirmed by Western blot and reverse transcription polymerase chain reaction analyses. It was concluded that treatment with a β3-adrenergic agonist stimulates UCP expression, which may lead to an increase in energy expenditure, and thereby is useful for the treatment and prevention of obesity in the dog.

【 授权许可】

Unknown