Journal of Veterinary Medical Science | |
Identification of Novel Immunogenic Proteins in Mycoplasma capricolum subsp. Capripneumoniae Strain M1601 | |
Xuan ZHANG1  Ying HE1  Ping ZHAO1  Hai-yan ZHAO1  Zhong-xin LU1  Nian-zhang ZHANG1  Peng-cheng GAO1  Ke-shan ZHANG1  Yue-feng CHU1  | |
[1] State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China | |
关键词: homology; identification; immunoblotting; immunogenic proteins; Mycoplasma capricolum subsp. Capripneumonia; | |
DOI : 10.1292/jvms.12-0095 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
References(26)Cited-By(4)The purpose of this study was to obtain immunogenic proteins and potential proteins of interest that were isolated from Mycoplasma capricolum subsp. capripneumoniae (Mccp) by MALDI-TOF mass spectrometry. One-dimensional SDS-PAGE and two-dimensional gel electrophoresis of whole cell preparation were conducted, and membrane proteome maps were prepared by immunoblotting. One-dimensional SDS-PAGE identified three immunogenic proteins with molecular masses in the range 29–97.2 kDa, two of which were in the membrane protein fraction. After two-dimensional gel electrophoresis, 20 highly immunogenic proteins were identified in the whole cell protein preparation while 9 immunogenic proteins were identified in the membrane protein fraction. This indicated that membrane proteins were the principle immunogenic proteins in Mccp. These proteins may have potential for the development of improved diagnostic tests and possible vaccines.
【 授权许可】
Unknown
【 预 览 】
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