期刊论文详细信息
Revista da Sociedade Brasileira de Medicina Tropical
Identification and purification of immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine (Leishvacin®)
Sandra Regina Afonso Cardoso1  João Carlos França Da Silva1  Roberto Teodoro Da Costa1  Wilson Mayrink1  Maria Norma Melo1  Marilene Suzan Marques Michalick1  Ibrahim Afrânio Willi Liu1  Ricardo Toshio Fujiwara1  Evaldo Nascimento1 
[1] ,Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de ParasitologiaBelo Horizonte MG ,Brasil
关键词: Tegumentary leishmaniasis;    immunogenic proteins;    Leishvacin;    Leishmaniose tegumentar;    proteínas imunogênicas;    Leishvacin®;   
DOI  :  10.1590/S0037-86822003000200001
来源: SciELO
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【 摘 要 】

Immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine against American tegumentary leishmaniasis (Leishvacin®), produced by Biobrás (Biochemistry of Brazil ), Montes Claros, State of Minas Gerais, Brazil, were identified and purified by polyacrylamide electrophoresis gel and electroelution. C57BL/10 mice were vaccinated with proteins with estimated molecular weights of 42, 46, 63, 66, 73, 87, 97, and 160kDa in three doses of 30µg of each protein at 15-day intervals combined with 250µg of Corynebacterium parvum followed by a challenge infection with 10(5) infective promastigotes from Leishmania (Leishmania) amazonensis. The ability of these proteins to induce immune response and protection was analyzed. No statistical difference was observed in the level of IFN-g induced by proteins in vaccinated groups in comparison with control groups. Six months after challenge infection, protection levels of 28.57; 42.86; 57.14; 42.86; 42.86, 57.14; 42.86 and 57.14% were demonstrated for each purified protein.

【 授权许可】

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 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License

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