Identification and purification of immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine (Leishvacin®)

Revista da Sociedade Brasileira de Medicina Tropical

Sandra Regina Afonso Cardoso¹  João Carlos França Da Silva¹  Roberto Teodoro Da Costa¹  Wilson Mayrink¹  Maria Norma Melo¹  Marilene Suzan Marques Michalick¹  Ibrahim Afrânio Willi Liu¹  Ricardo Toshio Fujiwara¹  Evaldo Nascimento¹ 

[1] ,Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de ParasitologiaBelo Horizonte MG ,Brasil

关键词: Tegumentary leishmaniasis;    immunogenic proteins;    Leishvacin;    Leishmaniose tegumentar;    proteínas imunogênicas;    Leishvacin®;   

DOI  :  10.1590/S0037-86822003000200001

来源: SciELO

【 摘 要 】

Immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine against American tegumentary leishmaniasis (Leishvacin®), produced by Biobrás (Biochemistry of Brazil ), Montes Claros, State of Minas Gerais, Brazil, were identified and purified by polyacrylamide electrophoresis gel and electroelution. C57BL/10 mice were vaccinated with proteins with estimated molecular weights of 42, 46, 63, 66, 73, 87, 97, and 160kDa in three doses of 30µg of each protein at 15-day intervals combined with 250µg of Corynebacterium parvum followed by a challenge infection with 10(5) infective promastigotes from Leishmania (Leishmania) amazonensis. The ability of these proteins to induce immune response and protection was analyzed. No statistical difference was observed in the level of IFN-g induced by proteins in vaccinated groups in comparison with control groups. Six months after challenge infection, protection levels of 28.57; 42.86; 57.14; 42.86; 42.86, 57.14; 42.86 and 57.14% were demonstrated for each purified protein.

【 授权许可】

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