Oligopeptide M13 Phage Display in Pathogen Research

Viruses

Jonas Kügler¹  Jonas Zantow¹  Torsten Meyer¹ 

[1] Technische Universität Braunschweig, Institut für Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie, Spielmannstr. 7, 38106 Braunschweig;

关键词: phage display;    ORF selection;    immunogenic proteins;    vaccine;    oligopeptide phage display;    diagnostic;    phage display libraries;    genome libraries;    antigen discovery;   

DOI  :  10.3390/v5102531

来源: mdpi

【 摘 要 】

Phage display has become an established, widely used method for selection of peptides, antibodies or alternative scaffolds. The use of phage display for the selection of antigens from genomic or cDNA libraries of pathogens which is an alternative to the classical way of identifying immunogenic proteins is not well-known. In recent years several new applications for oligopeptide phage display in disease related fields have been developed which has led to the identification of various new antigens. These novel identified immunogenic proteins provide new insights into host pathogen interactions and can be used for the development of new diagnostic tests and vaccines. In this review we focus on the M13 oligopeptide phage display system for pathogen research but will also give examples for lambda phage display and for applications in other disease related fields. In addition, a detailed technical work flow for the identification of immunogenic oligopeptides using the pHORF system is given. The described identification of immunogenic proteins of pathogens using oligopeptide phage display can be linked to antibody phage display resulting in a vaccine pipeline.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.