Applicability of In-House Loop-Mediated Isothermal Amplification for Rapid Identification of Mycobacterium tuberculosis Complex Grown on Solid Media

Japanese Journal of Infectious Diseases

Tetsu Mukai²  Sopa Srisungngam⁵  Supranee Bunchoo⁵  Benjawan Phetsuksiri⁵  Chie Nakajima¹  Yasuhiko Suzuki¹  Janisara Rudeeaneksin⁵  Dhanida Roienthong³  Shigeyuki Hamada⁴ 

[1] Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control;Leprosy Research Center, National Institute of Infectious Diseases;Bureau of Tuberculosis, Department of Disease Control, Ministry of Public Health;Research Collaboration Center for Emerging and Re-emerging Diseases, Osaka University;National Institute of Health, Department of Medical Sciences, Ministry of Public Health

关键词: tuberculosis;    solid culture;    identification;    LAMP;   

DOI  :  10.7883/yoken.66.249

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

References(18)A simple, rapid, and low-cost identification method is required in tuberculosis high-burden countries. We report the applicability of in-house loop-mediated isothermal amplification (LAMP) targeting 16S ribosomal RNA for the rapid identification of Mycobacterium tuberculosis complex grown on Lowenstein–Jensen media. Eighty acid-fast staining-positive clinical isolates were selected and used to evaluate the LAMP assay in comparison with polymerase chain reaction and conventional culture-based tests. The LAMP assay identified 60 M. tuberculosis isolates from 80 clinical isolates using simple heat-extracted DNA directly from the colony suspension. The results were in complete agreement with those obtained using the other methods, and the utility of the direct LAMP assay from a colony was demonstrated. The LAMP assay appears to be a practical and low-cost method that can be used for the rapid identification of M. tuberculosis isolates and suitable for endemic low-resource settings.

【 授权许可】

Unknown