Loop-Mediated Isothermal Amplification for Rapid Identification of Mycobacterium tuberculosis in Comparison with Immunochromatographic SD Bioline MPT64 Rapid® in a High Burden Setting

Japanese journal of infectious diseases

Janisara Rudeeaneksin¹  Sopa Srisungngam²  Benjawan Phetsuksiri³  Supranee Bunchoo⁴ 

[1] Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control;Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education(GI-CoRE), Hokkaido University;National Institute of Health, Department of Medical Sciences, Ministry of Public Health;Section of Bacterial Infections, Research Collaboration Center for Emerging and Re-emerging Infectious Diseases

关键词: tuberculosis;    identification;    LAMP;    immunochromatography;   

DOI  :  10.7883/yoken.JJID.2018.128

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

Loop-mediated isothermal amplification (LAMP) was assessed for rapid identification of Mycobacterium tuberculosis complex (MTC) in comparison with an immunochromatographic test (ICT) using SD Bioline Ag MPT64 Rapid®. One hundred and fifty-one MGIT cultures positive for acid-fast bacilli were tested for MTC. DNA was extracted from a small portion of culture samples by heat lysis and subjected to LAMP analysis. Of these, 144 were positive and 5 were negative by both tests. One culture that was ICT negative but was LAMP positive was confirmed to have a mutation in the mpt64 gene. The agreement was 98.68% (95% confidence interval [CI]: 94.80–99.77), and the kappa value was 0.83% (95% CI: 0.59–1.00). Good correlation results suggested that LAMP assay is a reliable molecular test for rapid identification of MTC and is practical for use in resource-limited, high burden settings.

【 授权许可】

Unknown