Attenuation of cell cycle progression by 2,3,7,8-tetrachlorodibenzo-p-dioxin eliciting ovulatory blockade in gonadotropin-primed immature rats

Endocrine Journal

Jy-Young Park²  Nak-Kyun Jung²  Sang-Young Chun²  Hwi-Cheul Lee¹  Sung-Yun Kim²  Sung-Woo Kim¹  Won-Kyong Chang¹  Jin-Ki Park¹  Jeong-Hoh Park² 

[1] Animal Biotechnology Division, National Institute of Animal Science, Suwon, Republic of Korea;Hormone Research Center and School of Biological Sciences & Technology, Chonnam National University, Kwangju, Republic of Korea

关键词: Dioxin;    AhR;    Ovary;    Cell cycle;   

DOI  :  10.1507/endocrj.K10E-220

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(37)Cited-By(5)2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) reduces ovulation rate in rats. The present study was to investigate whether TCDD alters the progression of cell cycle, and thus resulting in the blockade of ovulation in gonadotropin-primed, immature rats. The ovulation rate and ovarian weight were reduced in intact rats given TCDD (32 μg/kg BW in corn oil) by gavage one day before pregnant mare’s serum gonadotropin (PMSG; 5 IU/rat) injection. Flow cytometry demonstrated that the percentage of granulosa cells in S-phase was increased at 24 h following PMSG treatment, but declined at 8 h following hCG treatment in corn oil-treated rats. Interestingly, the number of S-phase cells in TCDD-treated rats was reduced 24 and 48 h following PMSG treatment. TCDD, however, increased the percentage of cells in G2/M-phase at 24 h following PMSG treatment. TCDD inhibited the mRNA levels of Cdk2 at 0 h and 24 h, and cyclin D2 at 24 h and 48 h following PMSG treatment. Protein levels of aryl hydrocarbon receptor in granulosa cells were elevated in TCDD-treated rats at 12 h and 24 h following PMSG treatment. The present study indicates that TCDD reduces S-phase cells and inhibits levels of Cdk2 and cyclin D2 at 24 h following PMSG treatment, implying the ovulation-inhibiting action of TCDD may be exerted through the attenuation of cell cycle progression via AhR-mediated cascade.

【 授权许可】

Unknown