Expression of E16/CD98LC/hLAT1 is responsive to 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin

FEBS Letters

Iseki, Atsushi¹  Sarkar, Devanand¹  Seo, Hisao¹  Kambe, Fukushi¹  Hirata, Akihiro¹  Ohmori, Sachiko¹ 

[1] Department of Endocrinology and Metabolism, Research Institute of Environmental Medicine, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan

关键词: cDNA representational difference analysis;    Dioxin;    Amino acid transporter E16;    Gene regulation;    Hepatocyte;   

DOI  :  10.1016/S0014-5793(99)01574-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We employed cDNA representational difference analysis to identify new genes that are upregulated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in a human hepatoblastoma cell line HepG2. We isolated several TCDD-responsive cDNAs. Sequence analysis revealed that one of them encodes E16/CD98LC/hLAT1, an integral membrane protein involved in multiple cellular functions including cellular transport of L-type amino acids. Northern blot analysis confirmed the TCDD-dependent upregulation of the mRNA. Induction of E16/CD98LC/hLAT1 mRNA by TCDD did not require de novo protein synthesis as revealed by the experiment using cycloheximide. Consistent with the changes at mRNA level, the transport of ³H-leucine into HepG2 cells was significantly increased by TCDD treatment. These findings provide a novel aspect of biological effects of TCDD on human hepatocytes.

【 授权许可】

Unknown