Dioxin induces a novel nuclear factor, DIF‐3, that is implicated in spermatogenesis

FEBS Letters

Iwata, Ryoko²  Kuroda, Masahiko²  Ohbayashi, Tetsuya²  Matsuda, Yoichi¹  Mukai, Kiyoshi²  Evine, Kazumi²  Fujii-Kuriyama, Yoshiaki¹  Isobe, Taku¹  Kameta, Akemi²  Mimura, Junsei¹  Oikawa, Kosuke² 

[1] Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Honchou 4-1-8, Kawaguchi, Saitama 332-0012, Japan;Department of Pathology, Tokyo Medical University, Shinjuku 6-1-1, Shinjuku-ku, Tokyo 160-8402, Japan

关键词: Dioxin inducible factor-3;    2;    3;    7;    8-Tetrachlorodibenzo-p-dioxin;    Aryl hydrocarbon receptor;    Zinc-finger protein;    AhR;    aryl hydrocarbon receptor;    TCDD;    2;    3;    7;    8-tetrachlorodibenzo-p-dioxin;    DIF-3;    dioxin inducible factor-3;    RDA;    cDNA representational difference analysis;   

DOI  :  10.1016/S0014-5793(01)03039-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD; dioxin), a member of a class of environmental pollutants represented by polychlorinated dibenzo-p-dioxins and dibenzofurans, is one of the most toxic artificial compounds ever developed. In this study, we identified a novel TCDD target gene, DIF-3 (dioxin inducible factor-3), by cDNA representational difference analysis. DIF-3 protein is a nuclear factor and possesses a zinc-finger motif at its N-terminus. High DIF-3 mRNA expression in the testes was demonstrated by Northern blot analysis and abundant DIF-3 protein was detected during spermatogenesis. Thus, these results suggest that DIF-3 may be a target gene mediating the reproductive toxicity induced by TCDD.

【 授权许可】

Unknown