Identification of Weakly Beta-Hemolytic Porcine Spirochetes by Biochemical Reactions, PCR-Based Restriction Fragment Length Polymorphism Analysis and Species-Specific PCR

Journal of Veterinary Medical Science

Masuo SUEYOSHI¹  Hiroshi ARAKI³  Tatsuo OHYA² 

[1] Department of Veterinary Hygiene, Faculty of Agriculture, Miyazaki University;Kyushu Research Station, Environmental/Enzootic Diseases Research Team, National Institute of Animal Health;Nagasaki Prefecture Kenhoku Livestock Hygiene Service Center

关键词: Brachyspira;    PCR-RFLP;    spirochete;   

DOI  :  10.1292/jvms.70.837

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)We examined the usefulness of PCR-based restriction fragment length polymorphism (PCR-RFLP) and species-specific PCR combined with a newly devised rapid biochemical test using microplates for identifying weakly beta-hemolytic intestinal spirochetes (WBHIS) isolated from pigs. WBHIS strains showing atypical biochemical characteristics were decisively identified at the species level by PCR-RFLP and species-specific PCR. Identification of WBHIS at the species level in routine diagnostic work will certainly contribute to clarifying the pathogenicity of WBHIS.

【 授权许可】

Unknown