Comparison of Mesenchymal Stem Cells Derived from Fat, Bone Marrow, Wharton’s Jelly, and Umbilical Cord Blood for Treating Spinal Cord Injuries in Dogs

Journal of Veterinary Medical Science

Hak-Hyun RYU¹  Yongsun KIM¹  Oh-Kyeong KWEON¹  Heung-Myong WOO²  Sung-Su PARK¹  Wan Hee KIM¹  Byung-Jae KANG¹  Gyu-Jin SUNG¹ 

[1] Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Daehak-dong, Gwanak-gu, Seoul 151–742, Korea;College of Veterinary Medicine and KNU Stem Cell Institute, Kangwon National University, Chuncheon 200–701, Korea

关键词: canine;    mesenchymal stem cell;    spinal cord injury;    transplantation;   

DOI  :  10.1292/jvms.12-0065

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(54)Cited-By(20)Previous animal studies have shown that transplantation of mesenchymal stem cells (MSCs) into spinal cord lesions enhances axonal regeneration and promotes functional recovery. We isolated the MSCs derived from fat, bone marrow, Wharton’s jelly and umbilical cord blood (UCB) positive for MSC markers and negative for hematopoietic cell markers. Their effects on the regeneration of injured canine spinal cords were compared. Spinal cord injury was induced by balloon catheter compression. Dogs with injured spinal cords were treated with only matrigel or matrigel mixed with each type of MSCs. Olby and modified Tarlov scores, immunohistochemistry, ELISA and Western blot analysis were used to evaluate the therapeutic effects. The different MSC groups showed significant improvements in locomotion at 8 weeks after transplantation (PP<0.05). Transplanted MSCs survived for 8 weeks and reduced IL-6 and COX-2 levels, which may have promoted neuronal regeneration in the spinal cord. Our data suggest that transplantation of MSCs promotes functional recovery after SCI. Furthermore, application of UCSCs led to more nerve regeneration, neuroprotection and less inflammation compared to other MSCs.

【 授权许可】

Unknown