Evaluation of the drug sensitivity and expression of 16 drugresistance-related genes in canine histiocytic sarcoma cell lines

Journal of Veterinary Medical Science

Koichi OHNO¹  Hajime TSUJIMOTO¹  Hirotaka TOMIYASU¹  Yuko GOTO-KOSHINO¹  Yasuhito FUJINO¹  Hajime ASADA¹ 

[1] Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Science, The University of Tokyo, Bunkyo-ku, Tokyo 113�?8657, Japan

关键词: canine;    drug resistance;    histiocytic sarcoma;    microtubule inhibitors;    TP53 gene;   

DOI  :  10.1292/jvms.14-0415

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(44)Cited-By(1)Supplementary materials(1)Canine histiocytic sarcoma (HS) is an aggressive tumor type originating from histiocyticcell lineages. This disease is characterized by poor response to chemotherapy and shortsurvival time. Therefore, it is of critical importance to identify and develop effectiveantitumor drugs against HS. The objectives of this study were to examine the drugsensitivities of 10 antitumor drugs. Using a real-time RT-PCR system, the mRNA expressionlevels of 16 genes related to drug resistance in 4 canine HS cell lines established fromdogs with disseminated HS were determined and compared to 2 canine lymphoma cell lines(B-cell and T-cell). These 4 canine HS cell lines showed sensitivities toward microtubuleinhibitors (vincristine, vinblastine and paclitaxel), comparable to those in the canineB-cell lymphoma cell line. Moreover, it was shown that P-gp in the HS cell lines used inthis study did not have enough function to efflux its substrate. Sensitivities tomelphalan, nimustine, methotrexate, cytarabine, doxorubicin and etoposide were lower inthe 4 HS cell lines than in the 2 canine lymphoma cell lines. The data obtained in thisstudy using cultured cell lines could prove helpful in the developing of advanced andeffective chemotherapies for treating dogs that are suffering from HS.

【 授权许可】

Unknown