GPCRとGタンパク質の結合の理解を目的とした�??GPCRアミノ酸配列の物理化学的性質の解析

Chem-Bio Informatics Journal

ギミ�? ガンガ�??D¹  美宅 成樹²  谷澤 英樹²  園山 正史² 

[1] 名古屋大学ベンチャービジネスラボラトリ;名古屋大学大学院工学研究科応用物理学分野

关键词: GPCR;    G-protein;    signal transduction;    proteomics;    bioinformatics;   

DOI  :  10.1273/cbij.8.49

学科分类：生物化学/生物物理

来源: Chem-Bio Informatics Society

【 摘 要 】

References(14)G-protein coupled receptors (GPCRs) bind with G-proteins upon activation by ligands. Understanding the mechanisms of specific binding between GPCRs and G-proteins is one of the most important issues in bioinformatics research. In this study, the physical properties of various regions were analyzed in order to classify GPCRs by G-protein family and to better understand binding specificity. We focused on cytoplasmic loops (IL1, IL2 and N/C-terminus of IL3), extracellular loops (NTL, EL1 and N/C-terminus of EL2) and cytoplasmic termini of transmembrane helices, except for helices that connect to C-terminus loops. The distribution of hydrophobicity, charge density, lysine and arginine densities, and loop length enabled discrimination of GPCRs with more than 90% accuracy.

【 授权许可】

Unknown