Distribution of IS629 and stx genotypesamong enterohemorrhagic Escherichia coli O157 isolates in YamaguchiPrefecture, Japan, 2004�?2013

Journal of Veterinary Medical Science

Mitsuhiro KAMEYAMA¹  Junko YABATA¹  Kiyoshi TOMINAGA¹  Yasuharu NOMURA¹ 

[1] Department of Health Science, Yamaguchi Prefectural Institute of Public Health and Environment, 2�?5�?67 Aoi, Yamaguchi 753�?0821, Japan

关键词: enterohemorrhagic;    Escherichia coli;    insertion sequence 629;    norV;    O157;    stx genotype;   

DOI  :  10.1292/jvms.15-0166

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)Patterns of insertion sequence (IS)629, norVgenotype, and Shiga toxin (Stx) genotype distribution were investigated amongst 203enterohemorrhagic Escherichia coli O157 isolates collected in YamaguchiPrefecture, Japan, between 2004 and 2013. A total of 114 IS629 patternswere identified; these were divided into eight IS groups (A–H). Ninety isolates carried anintact norV gene, whereas 113 isolates carried a norVwith a 204-bp deletion. Other than one isolate from IS group G, all isolates with anintact norV belonged to groups A–F, whereas isolates with a mutantnorV belonged to IS groups G and H. Seven stxgenotypes were identified, and of those, stx1a/stx2a waspredominant (n=105), followed by stx2c (n=32) and stx2a(n=27). The stx1a/stx2a genotype was associated with themutant norV isolates, whereas isolates with an intactnorV had the stx2c genotype. Therefore, certaincombinations of IS type and stx genotype appear to be more frequent amongO157 clades which may be useful for detection of predominant subtypes in the interest ofpublic health.

【 授权许可】

Unknown