3′‐end processing of precursor M1 RNA by the N‐terminal half of RNase E

FEBS Letters

Kim, Kwang-sun¹  Lee, Younghoon¹  Sim, Soyeong¹ 

[1] Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea

关键词: RNase E;    N-terminal half;    M1 RNA;    3′ processing;    Escherichia coli;   

DOI  :  10.1016/S0014-5793(02)03345-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

M1 RNA, the catalytic component of Escherichia coli RNase P, is derived from the 3′-end processing of precursor M1 RNA, a major transcript of the rnpB gene. In this study, we investigated the mechanism of 3′-end processing of M1 RNA using the recombinant N-terminal half RNase E. The cleavage site preference of RNase E differed from that of the 40% ammonium sulfate precipitate (ASP-40), a partially purified cell extract containing processing activity. However, the addition of a trace amount of ASP-40 changed the cleavage site preference of RNase E to that of ASP-40 suggesting the involvement of a soluble factor in cleavage site preference.

【 授权许可】

Unknown