Molecular and antimicrobial analyses of non-classical Bordetella isolated from a laboratory mouse

Journal of Veterinary Medical Science

Nur Hidayana MAHFODZ²  Siti Noraisah Ahmad NASRAH¹  Sazaly ABUBAKAR²  Shih Keng LOONG²  Siti Aisyah A. TALIB³  Haryanti Azura Mohamad WALI³  Pooi Fong WONG³ 

[1] Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia;Tropical Infectious Diseases Research & Education Centre, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia;Animal Experimental Unit, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia

关键词: Bordetella;    infectious disease;    laboratory mouse;    Malaysia;   

DOI  :  10.1292/jvms.15-0472

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(14)Cited-By(2)Accurate identification and separation of non-classical Bordetella species is very difficult. These species have been implicated in animal infections. B. hinzii, a non-classical Bordetella, has been isolated from mice in experimental facilities recently. We isolated and characterized one non-classical Bordetella isolate from the trachea and lung of an ICR mouse. Isolate BH370 was initially identified as B. hinzii by 16S ribosomal DNA and ompA sequencing. Additionally, isolate BH370 also displayed antimicrobial sensitivity profiles similar to B. hinzii. However, analyses of nrdA sequences determined its identity as Bordetella genogroup 16. The isolation of BH370 from a healthy mouse suggests the possibility of it being a commensal. The nrdA gene was demonstrated to possess greater phylogenetic resolution as compared with 16S ribosomal DNA and ompA for the discrimination of non-classical Bordetella species.

【 授权许可】

Unknown