Genotyping of Haemophilus Parasuis Isolated from Northwest China Using PCR-RFLP Based on the ompA Gene

Journal of Veterinary Medical Science

Ping ZHAO¹  Yin HE¹  Ji-Xing LIU¹  Nian-Zhang ZHANG¹  Peng-Chen GAO¹  Yue-Feng CHU¹  Zhong-Xin LU¹  Yong-Sheng LIU¹ 

[1] Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences

关键词: genotyping;    Haemophilus parasuis;    OmpA;    PCR-RFLP;   

DOI  :  10.1292/jvms.10-0296

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(14)Cited-By(3)Outer membrane proteins (OMPs) are the major virulent factors of Haemophilus parasuis. PCR-RFLP targeting the ompA gene was conducted to investigate the possibility of genotyping H. parasuis in this study. Fifteen reference strains and 49 isolates from pig farms in northwest China were genotyped by PCR-RFLP with a pair of specific primers. The results indicated that both the 15 reference strains and 49 isolates could be classified into 8 different genotypes by PCR-RFLP, respectively. Seven genotypes including AA, BB, BA, CA, BC, BD and CD existed simultaneously in the reference strains and isolates, but genotype CB only existed in the isolated strains. Interestingly, genotypes BA, CD and CA were only found in diseased pigs and accounted for 38.8%, 22.4% and 18.4% of the isolates, respectively. On the other hand, strains isolated from apparently healthy pigs were classified into genotypes AA, BB, BC and CB. However, the virulent reference serovar 1 strain has an AA genotype, and the fact that nearly all strains from the healthy pigs belonged to serovars classed as virulent suggests that these genotypes might also include virulent strains; therefore, further validation with more field strains is needed. The capability of the RFLP-PCR method based on the ompA gene for genotyping H. parasuis isolates indicates that this method may be a useful tool for epidemiological study.

【 授权许可】

Unknown