Intestinal parasites and genotyping of Giardia duodenalis in children: first report of genotype B in isolates from human clinical samples in Mexico

Memórias do Instituto Oswaldo Cruz

Julio César Torres-romero¹  Antonio De Jesus Euan-canto¹  Namibya Benito-gonzález¹  Nayely Padilla-montaño¹  Claribel Huchin-chan¹  Julio Lara-riegos¹  Roberto Cedillo-rivera¹ 

关键词: Giardia duodenalis;    genotyping;    PCR-RFLP;    tpi - gdh;    enteroparasites;   

DOI  :  10.1590/0074-0276140507

来源: SciELO

【 摘 要 】

Giardia duodenalis is one of the most prevalent enteroparasites in children. This parasite produces several clinical manifestations. The aim of this study was to determine the prevalence of genotypes of G. duodenalis causing infection in a region of southeastern Mexico. G. duodenalis cysts were isolated (33/429) from stool samples of children and molecular genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, targeting the triosephosphate isomerase ( tpi ) and glutamate dehydrogenase ( gdh ) genes. The tpi gene was amplified in all of the cyst samples, either for assemblage A (27 samples) or assemblage B (6 samples). RFLP analysis classified the 27 tpi -A amplicons in assemblage A, subgenotype I. Samples classified as assemblage B were further analysed using PCR-RFLP of the gdh gene and identified as assemblage B, subgenotype III. To our knowledge, this is the first report of assemblage B of G. duodenalis in human clinical samples from Mexico.

【 授权许可】

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