Astrocyte morphogenesis is dependent on BDNF signaling via astrocytic TrkB.T1

eLife

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[1] Department of Cell, Developmental, and Integrative Biology, School of Medicine, University of Alabama at Birmingham, Birmingham, United States;Department of Cell, Developmental, and Integrative Biology, School of Medicine, University of Alabama at Birmingham, Birmingham, United States;School of Neuroscience, Virginia Polytechnic and State University, Blacksburg, United States;School of Neuroscience, Virginia Polytechnic and State University, Blacksburg, United States;School of Neuroscience, Virginia Polytechnic and State University, Blacksburg, United States;Graduate Program in Translational Biology, Medicine, and Health, Virginia Polytechnic Institute and State University, Blacksburg, United States;

关键词: astrocyte;    BDNF;    TrkB;    development;    synaptogenesis;    morphogenesis;    Mouse;   

DOI  :  10.7554/eLife.44667

来源: publisher

【 摘 要 】

10.7554/eLife.44667.001Brain-derived neurotrophic factor (BDNF) is a critical growth factor involved in the maturation of the CNS, including neuronal morphology and synapse refinement. Herein, we demonstrate astrocytes express high levels of BDNF’s receptor, TrkB (in the top 20 of protein-coding transcripts), with nearly exclusive expression of the truncated isoform, TrkB.T1, which peaks in expression during astrocyte morphological maturation. Using a novel culture paradigm, we show that astrocyte morphological complexity is increased in the presence of BDNF and is dependent upon BDNF/TrkB.T1 signaling. Deletion of TrkB.T1, globally and astrocyte-specifically, in mice revealed morphologically immature astrocytes with significantly reduced volume, as well as dysregulated expression of perisynaptic genes associated with mature astrocyte function. Indicating a role for functional astrocyte maturation via BDNF/TrkB.T1 signaling, TrkB.T1 KO astrocytes do not support normal excitatory synaptogenesis or function. These data suggest a significant role for BDNF/TrkB.T1 signaling in astrocyte morphological maturation, a critical process for CNS development.

【 授权许可】

CC BY