Glial cell development in the vertebrate central nervous system

astrocyte;CNS;development;glial;oligodendrocyte;stem cell

Zhou, Qiao ; Anderson, David J.

University:California Institute of Technology

Department:Biology

关键词: astrocyte;    CNS;    development;    glial;    oligodendrocyte;    stem cell;   

Others  :  https://thesis.library.caltech.edu/1314/1/Abstract.pdf

美国|英语

来源: Caltech THESIS

【 摘 要 】

Neurons and glial cells are the two most fundamental cell types of the vertebrate central nervous system (CNS).While neurons are directly responsible for information processing via their electrical activities, glial cells play essential supportive roles.For example, oligodendroglia insulates axons, microglia performs immune functions, and astroglia maintains homeostasis of the entire CNS.Malfunction of glial cells causes numerous debilitating diseases directly (such as glial tumors), or indirectly by disrupting the normal functions of neurons that they support (as in multiple sclerosis).Despite their functional importance, relatively little is known about the development of vertebrate CNS glial cells.Focusing on the possibility that members of the basic helix-loop-helix (bHLH) transcription factors may play important roles in the development of vertebrate glial cells, similar to their functions in neurons, I searched for novel bHLH factors expressed in glial cells.A new family of bHLH factors was found and named Olig.Intriguingly, one member of this family, Olig2, is sequentially expressed first in motoneuron progenitors and later in the oligodendroglia.The sequence and expression pattern of Olig2 is highly conserved among different vertebrate species including fish, birds and mammals.To understand the role of Olig2 in oligodendroglia development, I ectopically expressed Olig2 singly or in combination with other factors in chick embryos.My result suggests that Olig2 can promote oligodendrocyte formation in the absence of neurogenic bHLH factors, which are negative regulators of glial fate.Other groups of researchers reported that in the presence of neurogenic factors, Olig2 promotes motoneuron development instead.Olig2 gene is therefore sufficient to specify the fate of either a neuronal subtype or a glial subtype, together with neurogenic factors.To further assess whether Olig genes are required for motoneuron and oligodendroglia development, I knocked out both Olig2 and Olig1 genes in mouse.In double null mutants, spinal motoneurons and oligodendroglia precursors from the entire CNS fail to develop, demonstrating that Olig genes are absolutely necessary for the generation of these cell types.Unexpectedly, in the absence of both Olig1 and Olig2, spinal motoneurons are transformed into V2 interneurons whereas oligodendroglial cells are respecified as astroglial cells.These results suggest that Olig genes are not involved in neuron-glia decision, but rather in specifying subtype identities of neuron and glia.Given that motoneurons and oligodendrocytes likely derive from common precursors, the expression of Olig may serve to couple the subtype identities of both neurons and glial cells sequentially generated from the same stem cells.The series of studies on Olig genes contributed on two areas of neural development.First, they shed important light on the specification of oligodendrocyte and astrocyte, the two major glial types in the vertebrate CNS.Second, they revealed that cell fate determinations of neuron and glia are not two unrelated events as often believed, on the contrary, they are deeply intertwined.