La biología molecular en la precisión diagnósticade las leucemias

Revista Cubana de Hematología, Inmunología y Hemoterapia

Amor Vigil, Ana María¹ 

[1] Instituto de Hematología e Inmunología, La Habana, Cuba

关键词: diagnostic;    leukemia;    molecular biology;    AML1-ETO;    BCR-ABL;    CBFβ;    -MYH11;    PML-RARα;    .;   

DOI  :  

学科分类：生理学与病理学

来源: SciELO

【 摘 要 】

Cytogenetic and molecular studies have correlated the presence of certain chromosomal and molecular anomalies with leukemia and lymphomas specific types. These evidences have allowed the progressive improvement of the system of classification of the oncohematological entities. Currently, the presence of certain cytogenetic or molecular anomalies is sufficient to identify specific entities and, in some occasions, the diagnostic changes after an integral analysis of cytomorfologic, cytogenetic and molecular studies. T This report aims to highlight the importance of molecular study when cytomorphology is complex and leads to erroneous diagnoses. Through polymerase chain reaction, prior reverse transcription of the RNA isolated from medullary blood, four biomarkers were studied: the fusion genes AML1-ETO, BCR-ABL, CBFβ-MYH11 and PML-RARα. Fourteen patients with initial diagnosis of: promyelocytic leukemia (n= 6), acute leukemia without lineage definition (n= 3) and chronic myeloid leukemia in myeloid blastic crisis or acute myeloid leukemia (AML) (n= 5) were studied. At the end of the molecular characterization all were diagnosed as AML. These results enlightened the role of the molecular studies in the classification of leukemia, which permit the patient receives the more appropriate treatment and achieve a better response.

【 授权许可】

CC BY-NC-SA