Combined study on clastogenic, aneugenic and apoptotic properties of doxorubicin in human cells in vitro

Journal of Biological Research

Vasiliki Chondrou¹ 

[1] University of Patras

关键词: Apoptosis-caspase-3;    Doxorubicin;    Fpg and hOGG1 comet assay;    Micronucleation;    Mitotic spindle disturbance;   

DOI  :  10.1186/s40709-018-0089-z

学科分类：生物科学（综合）

来源: Springer

【 摘 要 】

BackgroundDoxorubicin is a widely used anticancer drug due to its broad spectrum of antitumor activity. Various mechanisms have been proposed for its cytostatic activity, including DNA intercalation, topoisomerase II inhibition, generation of free radicals and apoptosis. The present study aims to further clarify the cytostatic activity of doxorubicin by its specific effect on (a) DNA damage, (b) micronucleation and (c) apoptosis, using a combination of different methods and cell systems such as human lymphocytes and HL-60 human leukemic cells. DNA lesions were analyzed by the alkaline comet assay in combination with formamidopyrimidine (Fpg) and human 8-oxoguanine (hOGG1) repair enzymes. Micronucleation was investigated by the Cytokinesis-Block Micronucleus assay (CBMN) in combination with Fluorescence In Situ Hybridization analysis. Impairment on mitotic apparatus was investigated by double immunofluorescence of β- and γ-tubulin. Apoptotic cell frequency was determined by the CBMN cytome assay. Complementary to the above, caspase-3 level was investigated by Western blot.

【 授权许可】

Free