Transcription initiation sites and promoter structure of the human TRAIL‐R3 gene1

FEBS Letters

Redondo, Juan Miguel²  Ruiz de Almodóvar, Carmen¹  López-Rivas, Abelardo¹  Rodrı́guez, Antonio² 

[1] Instituto de Parasitologı́a y Biomedicina, CSIC, calle Ventanilla 11, E-18001 Granada, Spain;Centro de Biologı́a Molecular Severo Ochoa, Universidad Autónoma de Madrid, E-28049 Madrid, Spain

关键词: TRAIL-R3;    Decoy receptor;    Promoter structure;    Transcription;    Apoptosis;    Doxorubicin;    TRAIL;    tumor necrosis factor-related apoptosis-inducing ligand;    TNF;    tumor necrosis factor;    GPI;    glycosylphosphatidylinositol;    AP-1;    activator protein 1;   

DOI  :  10.1016/S0014-5793(02)03544-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

TRAIL-R3 is a decoy receptor for TRAIL (tumor necrosis factor-related apoptosis-inducing ligand), a member of the tumor necrosis factor ligand family. In several cell types decoy receptors inhibit TRAIL-induced apoptosis by binding TRAIL and preventing its binding to TRAIL pro-apoptotic receptors. Here we report the cloning of the promoter region of human TRAIL-R3 and the mapping of the transcriptional start sites. This gene contains a consensus TATA box and the minimal promoter lies within the first 33 nucleotides upstream of the transcription start site. Transient transfection assays of luciferase reporter plasmids demonstrate that human TRAIL-R3 promoter can be induced in doxorubicin-treated MCF-7 cells in a p53-independent manner.

【 授权许可】

Unknown