Involvement of K + ATP and Ca 2+ channels in hydrogen sulfide-suppressed ageing of porcine oocytes

Biological research: BR

Jan Nevoral¹  Tereza Zalmanova²  Kristyna Hoskova³ 

[1] Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic;Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic;Institute of Animal Science, Prague 10, Czech Republic

关键词: Oocyte;    Gasotransmitter;    Hydrogen sulfide;    Ion channel;    Oocyte ageing;   

DOI  :  10.1186/s40659-018-0187-2

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Hydrogen sulfide has been shown to improve the quality of oocytes destined for in vitro fertilization. Although hydrogen sulfide is capable of modulating ion channel activity in somatic cells, the role of hydrogen sulfide in gametes and embryos remains unknown. Our observations confirmed the hypothesis that the KATP and L-type Ca2+ ion channels play roles in porcine oocyte ageing and revealed a plausible contribution of hydrogen sulfide to the modulation of ion channel activity. We confirmed the benefits of the activation and suppression of the KATP and L-type Ca2+ ion channels, respectively, for the preservation of oocyte quality. Our experiments identified hydrogen sulfide as promoting the desired ion channel activity, with the capacity to protect porcine oocytes against cell death. Further experiments are needed to determine the exact mechanism of hydrogen sulfide in gametes and embryos.

【 授权许可】

CC BY