FEBS Letters | |
Do voltage‐gated Kv1.1 and inward rectifier Kir2.1 potassium channels form heteromultimers? | |
Eggermont, Jan2  Daenens, Paul1  Nilius, Bernd2  Buyse, Gunnar2  Tytgat, Jan1  Droogmans, Guy2  | |
[1] University of Leuven, Laboratory of Toxicology, Van Evenstraat 4, B-3000 Leuven, Belgium;University of Leuven, Laboratory of Physiology, Campus Gasthuisberg, O & N, B-3000 Leuven, Belgium | |
关键词: Potassium channel; Ion channel; α-Subunit; Oocyte; | |
DOI : 10.1016/0014-5793(96)00674-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Possible heteromultimer formation between Kv- and Kir-type K+ channels was investigated, in connection with the known functional diversity of K+ channels in vivo. Voltage-clamp experiments were performed on Xenopus oocytes, either injected with concatenated Kir2.1-Kv1.1 mRNA, or co-injected with Kv1.1 and Kir2.1 mRNA. K+ currents could be approximated by the algebraic sum of the 2 K+ current types alone. The tandem construct did not show functional expression, although it could be detected by Western blotting. We conclude that Kv1.1 and Kir2.1 α-subunit proteins fail to assemble and do not contribute functional diversity to K+ channels.
【 授权许可】
Unknown
【 预 览 】
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