期刊论文详细信息
FEBS Letters
Do voltage‐gated Kv1.1 and inward rectifier Kir2.1 potassium channels form heteromultimers?
Eggermont, Jan2  Daenens, Paul1  Nilius, Bernd2  Buyse, Gunnar2  Tytgat, Jan1  Droogmans, Guy2 
[1] University of Leuven, Laboratory of Toxicology, Van Evenstraat 4, B-3000 Leuven, Belgium;University of Leuven, Laboratory of Physiology, Campus Gasthuisberg, O & N, B-3000 Leuven, Belgium
关键词: Potassium channel;    Ion channel;    α-Subunit;    Oocyte;   
DOI  :  10.1016/0014-5793(96)00674-6
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Possible heteromultimer formation between Kv- and Kir-type K+ channels was investigated, in connection with the known functional diversity of K+ channels in vivo. Voltage-clamp experiments were performed on Xenopus oocytes, either injected with concatenated Kir2.1-Kv1.1 mRNA, or co-injected with Kv1.1 and Kir2.1 mRNA. K+ currents could be approximated by the algebraic sum of the 2 K+ current types alone. The tandem construct did not show functional expression, although it could be detected by Western blotting. We conclude that Kv1.1 and Kir2.1 α-subunit proteins fail to assemble and do not contribute functional diversity to K+ channels.

【 授权许可】

Unknown   

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