The synaptic recruitment of lipid rafts is dependent on CD19‐PI3K module and cytoskeleton remodeling molecules

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: B cell activation;    BCR;    immunological synapse;   

DOI  :  10.1189/jlb.2A0614-287RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Sphingolipid‐andcholesterol‐richlipidraftmicrodomainsareimportantintheinitiationofBCRsignaling.AlthoughitisknownthatlipidraftspromotethecoclusteringofBCRandLynkinasemicroclusterswithintheBcellIS,themolecularmechanismoftherecruitmentoflipidraftsintotheBcellISisnotunderstoodcompletely.Here,wereportthatthesynapticrecruitmentoflipidraftsisdependentonthecytoskeleton‐remodelingproteins,RhoAandVav.Suchaneventisalsoefficientlyregulatedbymotorproteins,myosinIIAanddynein.Furtherevidencesuggeststhesynapticrecruitmentoflipidraftsis,byprinciple,aneventtriggeredbyBCRsignalingmoleculesandsecondmessengermolecules.BCR‐activatingcoreceptorCD19potentlyenhancessuchaneventdependingonitscytoplasmicTyr421andTyr482residues.TheenhancingfunctionoftheCD19‐PI3KmoduleinsynapticrecruitmentoflipidraftsisalsoconfirmedinhumanperipheralbloodBcells.Thus,theseresultsimproveourunderstandingofthemolecularmechanismoftherecruitmentoflipidraftmicrodomainsinBcellIS...

【 授权许可】

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