B cell receptor‐induced Ca2+ mobilization mediates F‐actin rearrangements and is indispensable for adhesion and spreading of B lymphocytes

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: B cell spreading;    membrane‐;    bound antigens;    B cell activation;    Ca2+ signal;    cofilin;   

DOI  :  10.1189/jlb.0312169

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Bcellsacquiremembrane‐boundcognateantigensfromthesurfaceoftheAPCsbyforminganIS,similartothatseeninTcells.Recognitionofmembrane‐boundantigensontheAPCsinitiatesadhesionofBlymphocytestotheantigen‐tetheredsurface,whichisfollowedbytheformationofradiallamellipodia‐likestructures,aprocessknownasBcellspreading.ThespreadingresponserequirestherearrangementofthesubmembraneactincytoskeletonandisregulatedmainlyviasignalstransmittedbytheBCR.Here,weshowthatcytoplasmiccalciumisaregulatorofactincytoskeletondynamicsinBlymphocytes.WefindthatBCR‐inducedcalciummobilizationisindispensibleforadhesionandspreadingofBcellsandthatPLCγandCRAC‐mediatedcalciummobilizationarecriticalregulatorsoftheseprocesses.Measuringcalciumandactindynamicsinlivecells,wefoundthatagenerationofactin‐basedmembraneprotrusionisstronglylinkedtothedynamicsofacytoplasmic‐freecalciumlevel.Finally,wedemonstratethatPLCγandCRACchannelsregulatetheactivityofactin‐severingproteincofilin,linkingBCR‐inducedcalciumsignalingtotheactindynamics...

【 授权许可】

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