Journal of Leukocyte Biology | |
B cell receptor-induced Ca2+ mobilization mediates F-actin rearrangements and is indispensable for adhesion and spreading of B lymphocytes | |
Nóra SzilÃgyi1  MÃté Maus4  David Medgyesi2  JÃnos Matkó and4  Gabriella SÃrmay4  Endre Kiss4  Andrea E. Schneider4  Ãgnes Enyedi3  | |
[1] Physiology and Neurobiology, Eötvös LórÃnd University, Budapest, Hungary; Department of Molecular Immunology, Max Planck Institute of Immunology, Freiburg, Germany;Department of Molecular Cell Biology, National Blood Center, Budapest, Hungary Physiology and Neurobiology, Eötvös LórÃnd University, Budapest, Hungary;Departments of Immunology and Physiology and Neurobiology, Eötvös LórÃnd University, Budapest, Hungary; | |
关键词: B cell spreading; membrane-bound antigens; B cell activation; Ca2+ signal; cofilin; | |
DOI : 10.1189/jlb.0312169 | |
学科分类:生理学 | |
来源: Federation of American Societies for Experimental Biology | |
【 摘 要 】
B cells acquire membrane-bound cognate antigens from the surface of the APCs by forming an IS, similar to that seen in T cells. Recognition of membrane-bound antigens on the APCs initiates adhesion of B lymphocytes to the antigen-tethered surface, which is followed by the formation of radial lamellipodia-like structures, a process known as B cell spreading. The spreading response requires the rearrangement of the submembrane actin cytoskeleton and is regulated mainly via signals transmitted by the BCR. Here, we show that cytoplasmic calcium is a regulator of actin cytoskeleton dynamics in B lymphocytes. We find that BCR-induced calcium mobilization is indispensible for adhesion and spreading of B cells and that PLCγ and CRAC-mediated calcium mobilization are critical regulators of these processes. Measuring calcium and actin dynamics in live cells, we found that a generation of actin-based membrane protrusion is strongly linked to the dynamics of a cytoplasmic-free calcium level. Finally, we demonstrate that PLCγ and CRAC channels regulate the activity of actin-severing protein cofilin, linking BCR-induced calcium signaling to the actin dynamics.
【 授权许可】
Unknown
【 预 览 】
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RO201912010183347ZK.pdf | 1172KB | download |