Induction of BAFF expression by IFN‐γ via JAK/STAT signaling pathways in human intestinal epithelial cells

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: B‐;    cell activating factor;    Interferon‐;    gamma;    HT‐;    29;   

DOI  :  10.1189/jlb.0412210

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

BAFFplaysanimportantroleinthedevelopmentofBcells.Here,weinvestigatedtheeffectofIFN‐γonBAFFexpressioninhumanintestinalepithelialcells.IFN‐γinducedsolubleandmembrane‐boundBAFFproductioninadose‐andtime‐dependentmanner.IFN‐γ‐inducedBAFFreleasefrompolarizedintestinalepithelialcellswasobservedinapicalandbasolateralcompartments.JAKIinhibitorsuppressedIFN‐γ‐inducedBAFFexpression.Moreover,IFN‐γenhancedSTAT1phosphorylationandexpressionofIRF‐1.TransienttransfectionandreportergeneassayshowedthattheBAFFpromoterregionspanning−750to−500bpfromthetranslationinitiationsitewascrucialforIFN‐γ‐inducedBAFFexpression.NucleotidesequenceanalysisrevealedaGASelementinthepromoterregion.ChIPassayconfirmedtheenhancedbindingofphosphorylatedSTAT1totheBAFFpromoterregionat−800to−601bp.Furthermore,IFN‐γenhancedDNAbindingtoGASanditstranscriptionalactivation,asdeterminedbytheEMSAandreportergeneassay.Collectively,theseresultssuggestthatIFN‐γinducesBAFFexpressioninhumanintestinalepithelialcellsthroughJAK/STATsignalingpathwaysthatmightactivatetheGASandIRF‐1‐bindingelementintheBAFFpromoter...

【 授权许可】

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