Dual retrovirus integration tagging: identification of new signaling molecules Fiz1 and Hipk2 that are involved in the IL‐7 signaling pathway in B lymphoblastic lymphomas

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: murine leukemia;    Stat5a;    B‐;    LBL;    Flt3;    CD43;   

DOI  :  10.1189/jlb.1109748

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

IL‐7R,FLT3,andCD43aresurfaceantigensexpressedduringthetransitionfrompro‐Btopre‐BcellsinBM.Tounderstandinteractionsbetweentheirsignalingpathways,weanalyzedspontaneousmouseB‐LBLswithdualMLVintegrationintoStat5aandFiz1orStat5aandHipk2.MLVintegrationresultedinup‐regulationofthesegenesinlymphomacellscomparedwithnormalpro‐BcellsfromtheBM.InlymphomaswithbothintegrationsintoStat5aandFiz1,increasesinphosphorylatedSTAT5Aandexpressionofc‐Myc,atargetgeneofSTAT5A,wereobservedfollowingstimulationoftheFLT3.CloneswiththedualintegrationsgrewfasterinIL‐7andFLT3L‐supplementedmediumthancloneswithStat5aintegrationalone.Ontheotherhand,inlymphomaswithintegrationsintoStat5aandHipk2,increasesinphosphorylatedSTAT5Aandexpressionofc‐Mycwereobservedfollowingcross‐linkingofCD43.Inconclusion,FLT3andCD43signalingpathwaysinvolveSTAT5AviaFiz1andHipk2inB‐LBLs.IdentificationofthedualMLVintegrationsitesinB‐LBLs,therefore,willprovideanexcellenttoolforidentificationofthesignalingpathwaysinB‐LBLs...

【 授权许可】

CC BY