期刊论文详细信息
Journal of Leukocyte Biology | |
Dual retrovirus integration tagging: identification of new signaling molecules Fiz1 and Hipk2 that are involved in the IL-7 signaling pathway in B lymphoblastic lymphomas | |
Kazuya Kanaya2  Tatsuaki Tsuruyama3  Tomoko Okuno3  Guang Jin1  Haruya Takeuchi3  Keiji Tamaki3  Yasuhiro Maruyama2  Hiroshi Hiai5  Tetsuya Takakuwa2  Takuya Hiratsuka4  Toshiaki Manabe4  Richard Kaszynski3  Yukiko Imai3  Munetaka Ozeki3  Takuro Nakamura1  | |
[1]Laboratory of Carcinogenesis, Cancer Institute, Tokyo, Japan | |
[2] and Laboratory of Carcinogenesis, Cancer Institute, Tokyo, Japan | |
[3] and Laboratory of Carcinogenesis, Cancer Institute, Tokyo, Japan | |
[4] andDepartment of Human Health Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan | |
[5] Department of Human Health Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan | |
[6] Department of Human Health Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan | |
[7] | |
[8]Department of Forensic Medicine and Molecular Pathology, Department of Forensic Medicine and Molecular Pathology, Department of Forensic Medicine and Molecular Pathology, | |
[9]Department of Diagnostic Pathology, and Department of Diagnostic Pathology, and Department of Diagnostic Pathology, and | |
[10]Shiga Medical Center Research Institute, Shiga, Japan Shiga Medical Center Research Institute, Shiga, Japan Shiga Medical Center Research Institute, Shiga, Japan | |
关键词: murine leukemia; Stat5a; B-LBL; Flt3; CD43; | |
DOI : 10.1189/jlb.1109748 | |
学科分类:生理学 | |
来源: Federation of American Societies for Experimental Biology | |
【 摘 要 】
IL-7R, FLT3, and CD43 are surface antigens expressed during the transition from pro-B to pre-B cells in BM. To understand interactions between their signaling pathways, we analyzed spontaneous mouse B-LBLs with dual MLV integration into Stat5a and Fiz1 or Stat5a and Hipk2. MLV integration resulted in up-regulation of these genes in lymphoma cells compared with normal pro-B cells from the BM. In lymphomas with both integrations into Stat5a and Fiz1, increases in phosphorylated STAT5A and expression of c-Myc, a target gene of STAT5A, were observed following stimulation of the FLT3. Clones with the dual integrations grew faster in IL-7 and FLT3L-supplemented medium than clones with Stat5a integration alone. On the other hand, in lymphomas with integrations into Stat5a and Hipk2, increases in phosphorylated STAT5A and expression of c-Myc were observed following cross-linking of CD43. In conclusion, FLT3 and CD43 signaling pathways involve STAT5A via Fiz1 and Hipk2 in B-LBLs. Identification of the dual MLV integration sites in B-LBLs, therefore, will provide an excellent tool for identification of the signaling pathways in B-LBLs.【 授权许可】
Unknown
【 预 览 】
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RO201912010182790ZK.pdf | 43KB | download |