Ethanol Inhibits Gastric Acid Secretion in Rats Through Increased AMP-Kinase Activity

Cellular Physiology and Biochemistry

关键词: Stomach;    Parietal cell;    H;    K-ATPase;    pH;    BCECF;    Alcohol;   

DOI  :  10.1159/000276553

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

The effects of ethanol on gastric acid secretion remain controversial. The present study examines the effect of low-dose (2%) short term (15-20min) ethanol exposure on gastric acid secretion via a potential interaction with AMP-activated protein kinase (AMPK). Real-time fluorescence digital imaging was used to provide functional evidence for the interaction of ethanol and AMPK in modulating secretagogue-induced acid secretion. Individual rat gastric glands were loaded with the pH-sensitive dye BCECF and the secretagogues carbachol (200 µM) or histamine (200 µM) were added to induce secretion. Rates of pH recovery were calculated as ΔpH_i/Δt. In one series of experiments, secretagogue-induced acid secretion was inhibited by 2% ethanol, or the AMPK activator AICAR monophosphate (AICAR) (20 mM). In a separate series, 2% ethanol was added in combination with compound C (20 µM), an AMPK inhibitor, to prevent activation of AMPK. 2% ethanol significantly suppressed stimulated acid secretion. In order to confirm modulation of AMPK activity by ethanol, the specific AMPK inhibitor compound C was used, which reversed the inhibitory effects of ethanol on stimulated acid secretion. This study demonstrates that low dose ethanol (2%) inhibits secretagogue-dependent acid secretion by activation of the AMPK pathway in rat gastric parietal cells.

【 授权许可】

CC BY-NC-ND