Sequence motif in control regions of the H+/K+ ATPase α and β subunit genes recognized by gastric specific nuclear protein(s)

FEBS Letters

Nishi, Tsuyoshi²  Futai, Masamitsu²  Oshiman, Ko-Ichi²  Tamura, Shigehiko²  Maeda, Masatomo²  Mori, Masaharu¹ 

[1] Department of Pathology, Okayama University Medical School, Okayama 700, Japan;Department of Organic Chemistry and Biochemistry, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567, Japan

关键词: H+/K+-ATPase;    Gastric specific transcription;    Gastric proton pump;    Parietal cell;    DNA binding protein;    HEPES;    4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;    EDTA;    ethylenediamine-tetraacetic acid;   

DOI  :  10.1016/0014-5793(92)80040-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A nuclear protein(s) from rat or pig stomach recognized a conserved sequence in the 5′-upstream regions of the rat and human H⁺/K⁺-ATPase α subunit genes. A gel retardation assay suggested that part of the binding site was located in the TAATCAGCTG sequence. No nuclear proteins capable of the binding could be detected in other tissues of rat (liver, brain, kidney, spleen and lung) or pig liver. The sequence motif (GATAGC) located 5′-upstream of the β-subunit gene also seemed to be recognized by the same protein, because the binding of nuclear protein to the sequence motifs in the α and β subunits was mutually competitive. Considering the sense-strand sequence of the binding motif in the α-subunit gene, we conclude that (G/C)PuPu(G/C)NGAT(A/T)PuPy is a core sequence motif for the gastric specific DNA binding protein (PCSF, parietal cell specific factor).

【 授权许可】

Unknown