Control region and gastric specific transcription of the rat H+,K+‐ATPase α subunit gene

FEBS Letters

Shimada, Akira¹  Futai, Masamitsu¹  Oshiman, Ko-Ichi¹  Tamura, Shigehiko¹  Maeda, Masatomo¹  Motojima, Kiyoto¹  Mahmood, Saleh¹ 

[1] Department of Organic Chemistry and Biochemistry, Institute of Scientific and Industrial Research, Osaka University, Osaka 567, Japan

关键词: H+;    K+-ATPase;    Gastric specific transcription;    Control region;   

DOI  :  10.1016/0014-5793(91)80404-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rat gastric H⁺,K⁺-ATPase α subunit gene was cloned and the nucleotide sequence of its 5′-upstream region was determined. Sequence comparison with the corresponding part of the human gene indicated the presence of highly conserved regions which may be important for specific transcription of the α subunit in gastric parietal cells. The amino-terminal sequence (Met-Gly-Lys-Ala-Glu-) of the rat enzyme was similar to those of the pig and human enzymes. The gene organization of the rat enzyme was also similar to that of the human gene: introns 1, 2 and 9 were located in exactly the same positions as those in the human gene, and, as in the latter, exon 6 was not separated by an intron. The sequences of introns 1 and 2 were highly conserved among the rat, human and pig genes, but were entirely different from those of Na⁺,K⁺-ATPase catalytic subunit genes. Northern blot hybridization indicated that the gene was transcribed only in gastric mucosa.

【 授权许可】

Unknown