Genes for members of the GATA‐binding protein family (GATA‐GT1 and GATA‐GT2) together with H+/K+‐ATPase are specifically transcribed in gastric parietal cells

FEBS Letters

Mushiake, Sotaro¹  Shimada, Shoichi²  Hasebe, Masahisa³  Futai, Masamitsu³  Maeda, Masatomo³  Tohyama, Masaya²  Etani, Yuri¹ 

[1] Department of Pediatrics, Osaka University Medical School, Suita, Osaka 565, Japan;Department of Anatomy and Neuroscience, Osaka University Medical School, Suita, Osaka 565, Japan;Department of Organic Chemistry and Biochemistry, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567, Japan

关键词: Parietal cell;    Gastric specific transcription;    GATA factor;    DNA-binding protein;    Hybridization (in situ);    H+/K+-ATPase;    bp;    base pair;    NaPB;    0.1 M sodium phosphate buffer pH 7.4;   

DOI  :  10.1016/0014-5793(94)80184-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

mRNAs for novel DNA-binding proteins (GATA-GT1 and GATA-GT2) recognizing the (G/C)PuPu(G/C)NGAT(A/T)PuPy sequence and H⁺/K⁺-ATPase (proton pump) α subunit were detected in parietal cells of the rat gastric body mucosa by in situ hybridization. These results suggest that GATA-GT1 and GATA-GT2 together with H⁺K⁺-ATPase are transcribed specifically in gastric parietal cells and that the two DNA-binding proteins may have important roles in cell specific gene regulation. Furthermore, we could detect parietal cells in different states of gene expression.

【 授权许可】

Unknown