FEBS Letters | |
Gastric GATA‐6 DNA‐binding protein: proteolysis induced by cAMP | |
Futai, Masamitsu2  Maeda, Masatomo1  Nakagawa, Reiko1  Sato, Ryuichiro1  Yokosawa, Hideyoshi3  | |
[1] Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565, Japan;Division of Biological Sciences, The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567, Japan;Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060, Japan | |
关键词: GATA factor; DNA-binding protein; Proteolysis; Proteasome; cAMP; A kinase; CHO; Chinese hamster ovary; dbcAMP; dibutyryl cAMP; dbcGMP; dibutyryl cGMP; DOG; sn-1; 2-dioctanoylglycerol; IP3; d-myo-Inositol-1; 4; 5-triphosphate; ECL; enhanced chemiluminescence; MG101; N-acetyl-Leu-Leu-norleucinal; MG115; benzyloxycarbonyl-Leu-Leu-norvalinal; TPA; 12-O-tetradecanoylphorbol 13-acetate; PSI; benzyloxycarbonyl-Ile-Glu(O-t-Bu)-Ala-leucinal; | |
DOI : 10.1016/S0014-5793(97)00443-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The rat gastric GATA DNA-binding protein, GATA-6 (GATA-GT1), was stably expressed in CHO-K1 cells. The GATA-6 protein was localized in the nucleus but not in the cytoplasm. Interestingly, when cells were treated with dibutyryl cAMP, the GATA-6 protein was specifically degraded. Such a phenomenon was not observed in the presence of 5′-AMP or dibutyryl cGMP. The cellular level of the GATA-6 protein was restored upon removal of dibutyryl cAMP. Degradation was also induced by cholera toxin, which increased the cellular cAMP concentration, and was inhibited by a protein kinase A inhibitor. However, activators of protein kinase C did not have any effect. The degradation was inhibited by proteasome inhibitors (PSI (benzyloxycarbonyl-Ile-Glu(O-t-Bu)-Ala-leucinal) and MG115 (benzyloxycarbonyl-Leu-Leu-norvalinal)) but not by those of lysosomes and serine proteases. These results suggest that a kinase-mediated protein phosphorylation is the cellular signal for degradation of the GATA-6 protein. This finding constitutes a novel aspect of regulation by GATA DNA-binding proteins, which are essential for developmental processes and tissue-specific transcription.
【 授权许可】
Unknown
【 预 览 】
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