Euphol from Euphorbia tirucalli Negatively Modulates TGF-β Responsiveness via TGF-β Receptor Segregation inside Membrane Rafts

PLoS One

Yu-Chen Kao¹  Tsai-Hui Duh¹  Ming-Wei Lin¹  Deng-Chyang Wu²  Yaw-Bin Huang²  Chun-Lin Chen²  Fang-Rong Chang³  Wei-Chiang Wu³  Ying-Pin Chen⁴  Pei-Hua Yang⁵ 

[1] Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC;Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan, ROC;Department of Pharmacy, School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC;Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung, Taiwan, ROC;Graduate Institute of Nature Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC

关键词: Lipids;    Cell membranes;    Cholesterol;    Lipid signaling;    Luciferase;    Phosphorylation;    Nystatin;    SMAD signaling;   

DOI  :  10.1371/journal.pone.0140249

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Transforming growth factor-β (TGF-β) responsiveness in cultured cells can be modulated by TGF-β partitioning between lipid raft/caveolae- and clathrin-mediated endocytosis pathways. Lipid rafts are plasma membrane microdomains with an important role in cell survival signaling, and cholesterol is necessary for the lipid rafts’ structure and function. Euphol is a euphane-type triterpene alcohol that is structurally similar to cholesterol and has a wide range of pharmacological properties, including anti-inflammatory and anti-cancer effects. In the present study, euphol suppressed TGF-β signaling by inducing TGF-β receptor movement into lipid-raft microdomains and degrading TGF-β receptors.

【 授权许可】

CC BY