Kinome analyses of inflammatory responses to swine barn dust extract in human bronchial epithelial and monocyte cell lines:

Innate Immunity

SabariNath Neerukonda¹ 

关键词: Pro-inflammatory cytokines;    kinome;    swine barn dust;    signaling;    lung epithelial;    monocyte;   

DOI  :  10.1177/1753425918792070

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Exacerbated inflammation upon persistent barn organic dust exposure is a key contributor to the pathogenesis of lung inflammation and lung function decline. Barn dust constituents and the mechanisms contributing to the exacerbated inflammation are not clearly known. We set out to understand the inflammatory effects of Swine Barn Dust Extracts (SBDE) on human lung epithelial (BEAS2B) and macrophage (THP-1 monocyte derived) cell lines on a kinome array to determine phosphorylation events in the inflammatory signaling pathways. Upon identifying events unique to SBDE or those induced by innate immune ligands in each cell line, we validated the signaling pathway activation by transcriptional analyses of downstream inflammatory cytokines. Our findings indicate that SBDE-mediated pro-inflammatory effects are predominantly due to the induction of neutrophilic chemokine IL-8. Differentially phosphorylated peptides implicated in IL-8 induction in BEAS2B cell line include, TLR2, 4, 5, 7, 8, 9, PKC, MAP kinases (p38,...

【 授权许可】

CC BY