Enterococcus faecalis promotes osteoclastogenesis and semaphorin 4D expression:

Innate Immunity

ShuaiWang¹ 

关键词: Enterococcus faecalis;    MAPK signaling pathways;    osteoclasts;    receptor activator of nuclear factor-kappa B lig;    tartrate-resistant acid phosphatase;   

DOI  :  10.1177/1753425915593162

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Enterococcus faecalis is considered a major bacterial pathogen implicated in endodontic infections and contributes considerably to periapical periodontitis. This study aimed to investigate the potential mechanisms by which E. faecalis accounts for the bone destruction in periapical periodontitis in vitro. Osteoclast precursor RAW264.7 cells were treated with E. faecalis ATCC 29212 and a wild strain of E. faecalis derived clinically from an infected root canal. The results showed that, to some extent, E. faecalis induced the RAW264.7 cells to form tartrate-resistant acid phosphatase (TRAP)-positive multinucleated osteoclast-like cells. This pathogen markedly stimulated RAW264.7 cells to express semaphorin 4D (Sema4D), which inhibits bone formation. Once RAW264.7 cells were primed by low-dose receptor activator of nuclear factor-kappa B ligand (RANKL), E. faecalis could significantly increase the production of TRAP-positive multinucleated cells and up-regulate the expression of osteoclast-specific markers, ...

【 授权许可】

CC BY