Scaling up of Lactic Acid Fermentation using Enterococcus faecalis

11th Curtin University Technology, Science and Engineering (CUTSE) International Conference

工业技术（总论）

Nolasco-Hipolito, Cirilo^1^2 ; Carvajal-Zarrabal, Octavio^3 ; Kelvin, Eivo^1 ; Tan, Yie Hua^4 ; Kohei, Mizuno^5 ; Nyoel, Stanley Anthony^1 ; Shoji, Esaki^6 ; Dieng, Hamady^7 ; Bujang, Kopli^8

Institute of Biotechnology, Universidad Del Papaloapan, Circuito Central, 200, Col. Parque Industrial, Tuxtepec, Oaxaca

CP. 68301, Mexico^1

Faculty of Engineering, Universiti Malaysia Sarawak, Sarawak, Kota Samarahan

94300, Malaysia^2

Biochemical and Nutrition Chemistry Area, University of Veracruz, SS Juan Pablo II s/n, Boca del Rio, Veracruz

CP 94294, Mexico^3

Department of Chemical Engineering, Curtin University, CDT 250, Sarawak, Miri

98009, Malaysia^4

Department of Creative Engineering, National Institute of Technology, Kitakyushu College, Kitakyushu

802-0985, Japan^5

Department of Control and Information Systems Engineering, Kurume National College of Technology, 1-1-1 Komorino, Kurume-shi, Fukuoka

830-8555, Japan^6

Institute of Biodiversity and Environmental Conservation, Universiti Malaysia Sarawak, Sarawak, Kota Samarahan

94300, Malaysia^7

Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Sarawak, Kota Samarahan

94300, Malaysia^8

关键词: Enterococcus faecalis;    Glucose consumption;    Industrial potentials;    Lactic acid bacteria;    Lactic acid fermentation;    Lactic acid production;    Process simulations;    Thermophilic strains;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/495/1/012049/pdf
DOI  :  10.1088/1757-899X/495/1/012049

学科分类：工业工程学

来源: IOP

【 摘 要 】

Lactic Acid bacterium (LAB) Enterococcus faecalis was used with the aim of scaling up a lactic acid fermentation (LAF) from 3L jar fermenter to pilot scale up to 100-L in an open fermentation. Batch mode in Lab and pilot scale fermentations were compared in terms of productivity, overall yield, cell growth, lactic acid production, and glucose consumption. Fermentations were performed at pH 6.86, 45 oC, and 100 rpm. Process simulation was conducted by using SuperPro Software and the data obtained from pilot scale. The results were compared using t-test and ANOVA. The results showed that the production rate of Lab scale was 4.96±0.09 g/Lh with a yield of 0.93 ±0.005. While for pilot scale, the production rate was 3.91 g/Lh with a yield of 0.915 ±0.005. There was no significant difference between both processes, regarding the yield, but there was a difference regarding the production rate, being slowly lower for pilot scale, but still high for industrial scaling up. Contamination was controlled fairly because Enterococcus faecalis is a thermophilic strain and then, proved to have industrial potential to produce Lactic Acid (LA).