期刊论文详细信息
卷:204
Certain (-)-epigallocatechin-3-gallate (EGCG) auto-oxidation products (EAOPs) retain the cytotoxic activities of EGCG
Wei, Yaqing ; Chen, Pingping ; Ling, Tiejun ; Wang, Yijun ; Dong, Ruixia ; Zhang, Chen ; Zhang, Longjie ; Han, Manman ; Wang, Dongxu ; Wan, Xiaochun ; Zhang, Jinsong
Anhui Agr Univ
关键词: EGCG;    Auto-oxidation;    Cytotoxic activity;    Sulfhydryl group;    Hydrogen peroxide;   
DOI  :  10.1016/j.foodchem.2016.02.134
学科分类:食品科学和技术
PDF
【 摘 要 】

(-)-Epigallocatechin-3-gallate (EGCG) from green tea has anti-cancer effect. The cytotoxic actions of EGCG are associated with its auto-oxidation, leading to the production of hydrogen peroxide and formation of numerous EGCG auto-oxidation products (EAOPs), the structures and bioactivities of them remain largely unclear. In the present study, we compared several fundamental properties of EGCG and EAOPs, which were prepared using 5 mg/mL EGCG dissolved in 200 mM phosphate buffered saline (pH 8.0 at 37 degrees C) and normal oxygen partial pressure for different periods of time. Despite the complete disappearance of EGCG after the 4-h auto-oxidation, 4-h EAOPs gained an enhanced capacity to deplete cysteine thiol groups, and retained the cytotoxic effects of EGCG as well as the capacity to produce hydrogen peroxide and inhibit thioredoxin reductase, a putative target for cancer prevention and treatment. The results indicate that certain EAOPs possess equivalent cytotoxic activities to EGCG, while exhibiting simultaneously enhanced capacity for cysteine depletion. These results imply that EGCG and EAOPs formed extracellularly function in concert to exhibit cytotoxic effects, which previously have been ascribed to EGCG alone. (C) 2016 Elsevier Ltd. All rights reserved.

【 授权许可】

   

【 预 览 】
附件列表
Files Size Format View
JA201706070002085SK.pdf KB PDF download
  文献评价指标  
  下载次数:15次 浏览次数:32次