Combination of Low Concentration of (−)-Epigallocatechin Gallate (EGCG) and Curcumin Strongly Suppresses the Growth of Non-Small Cell Lung Cancer in Vitro and in Vivo through Causing Cell Cycle Arrest

International Journal of Molecular Sciences

Dong-Hu Zhou²  Xuemin Wang²  Mingmin Yang²  Xiaoyan Shi²  Wenbin Huang¹ 

[1]Department of Pathology, Affiliated Nanjing First Hospital of Nanjing Medical University, Nanjing 210006, Jiangsu, China

[2] E-Mail:

[3]Department of Nutrition and Food Safety, Nanjing Medical University, Nanjing 211166, Jiangsu, China

[4] E-Mails:

关键词: EGCG;    curcumin;    cell cycle arrest;    combination;    chemoprevention;   

DOI  :  10.3390/ijms140612023

来源: mdpi

【 摘 要 】

(−)-Epigallocatechin gallate (EGCG) and curcumin are two naturally derived agents that have been widely investigated worldwide. They exhibit their anti-tumor effects in many types of cancers. In the current study, the effect of the combination of the two agents on non-small cell lung cancer (NSCLC) cells was investigated. The results revealed that at low concentrations, the combination of the EGCG and curcumin strongly enhanced cell cycle arrest. Flow cytometry analysis showed that the cells were arrested at G1 and S/G2 phases. Two main cell cycle related proteins cyclin D1 and cyclin B1 were significantly inhibited at the present of EGCG and curcumin. EdU (5-ethynyl-2′-deoxyuridine) fluorescence staining showed that the DNA replication was significantly blocked. A clonal growth assay also confirmed a marked repression of cell growth. In a lung cancer xenograft node mice model, combination of EGCG and curcumin exhibited protective effect against weight loss due to tumor burden. Tumor growth was strongly repressed by the combination of the two agents, without causing any serious side-effect. Overall, these results strongly suggest that EGCG in combination with curcumin could be a candidate for chemoprevention agent of NSCLC.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland