Sulforaphane effects on postinfarction cardiac remodeling in rats: modulation of redox-sensitive prosurvival and proapoptotic proteins

卷:34

Fernandes, Rafael Oliveira ; De Castro, Alexandre Luz ; Poletto Bonetto, Jessica Hellen ; Ortiz, Vanessa Duarte ; Muller, Dalvana Daneliza ; Campos-Carraro, Cristina ; Barbosa, Silvia ; Neves, Laura Maui ; Xavier, Leder Leal ; Schenkel, Paulo Cavalheiro ; Singal, Pawan ; Khaper, Neelam ; da Rosa Araujo, Alex Sander ; Bello-Klein, Adriane

Univ Fed Rio Grande do Sul

关键词: Myocardial infarction;    Heart failure;    Isothiocyanate;    Heme oxygenase-1;    Oxidative stress;    Autophagy;   

DOI  :  10.1016/j.jnutbio.2016.05.004

学科分类：食品科学和技术

【 摘 要 】

This study investigated whether sulforaphane (SFN), a compound found in cruciferous vegetables, could attenuate the progression of post-myocardial infarction (MI) cardiac remodeling. Male Wistar rats (350 g) were allocated to four groups: SHAM (n=8), SHAM + SFN (n=7), MI (n=8) and MI + SFN (n=5). On the third day after surgery, cardiac function was assessed and SFN treatment (5 mg/kg/day) was started. At the end of 25 days of treatment, cardiac function was assessed and heart was collected to measure collagen content, oxidative stress and protein kinase. MI and MI + SFN groups presented cardiac dysfunction, without signs of congestion. Sulforaphane reduced fibrosis (2.1-fold) in infarcted rats, which was associated with a slight attenuation in the cardiac remodeling process. Both infarcted groups presented increases in the oxidative markers xanthine oxidase and 4-hydroxinonenal, as well as a parallel increase in the antioxidant enzymes glutathione peroxidase and superoxide dismutase. Moreover, sulforaphane stimulated the cytoprotective heme oxygenase-1 (HO-1) (38%). Oxidative markers correlated with ERK 1/2 activation. In the MI + SFN group, up-regulation of ERK 1/2 (34%) and Akt (35%), as well as down-regulation of p38 (52%), was observed. This change in the prosurvival kinase balance in the MI + SFN group was related to a down-regulation of apoptosis pathways (Bax/Bcl-2/caspase-3). Sulforaphane was unable to modulate autophagy. Taken together, sulforaphane increased HO-1, which may generate a redox environment in the cardiac tissue favorable to activation of prosurvival and deactivation of prodeath pathways. In conclusion, this natural compound contributes to attenuation of the fibrotic process, which may contribute to mitigation against the progression of cardiac remodeling postinfarction. (C) 2016 Elsevier Inc. All rights reserved.