Migration channels produced by laser ablation for substrate endothelialization

Bulletin of the Polish Academy of Sciences. Technical Sciences

M. KotFaculty of Mechanical Engineering and Robotics, AGH University of Science and Technology, 30 Mickiewicza Ave., 30-059 Cracow, PolandOther articles by this author:De Gruyter OnlineGoogle Scholar¹  K. MaksymowInstitute of Metallurgy and Materials Science of Polish Academy of Sciences, 25 Reymonta St., 30-059 Cracow, PolandOther articles by this author:De Gruyter OnlineGoogle Scholar²  R. MajorInstitute of Metallurgy and Materials Science of Polish Academy of Sciences, 25 Reymonta St., 30-059 Cracow, PolandOther articles by this author:De Gruyter OnlineGoogle Scholar²  B. MajorInstitute of Metallurgy and Materials Science of Polish Academy of Sciences, 25 Reymonta St., 30-059 Cracow, PolandOther articles by this author:De Gruyter OnlineGoogle Scholar²  J. MarczakInstitute of Optoelectronics, Military University of Technology, 2 Kaliskiego St., 00-908 Warsaw, PolandOther articles by this author:De Gruyter OnlineGoogle Scholar³  J.M. LacknerJoanneum Research Forschungs-GmbH, Materials – Functional Surfaces, 94 Leobner Strasse, Leoben, A-8712 Niklasdorf, AustriaOther articles by this author:De Gruyter OnlineGoogle Scholar⁴ 

[1] Faculty of Mechanical Engineering and Robotics, AGH University of Science and Technology, 30 Mickiewicza Ave., 30-059 Cracow, Poland;Institute of Metallurgy and Materials Science of Polish Academy of Sciences, 25 Reymonta St., 30-059 Cracow, Poland;Institute of Optoelectronics, Military University of Technology, 2 Kaliskiego St., 00-908 Warsaw, Poland;Joanneum Research Forschungs-GmbH, Materials – Functional Surfaces, 94 Leobner Strasse, Leoben, A-8712 Niklasdorf, Austria

关键词: Keywords: : inorganic coatings;    pulsed laser ablation;    tissue scaffold;    endothelial cells migration.;   

DOI  :  10.2478/v10175-012-0045-2

学科分类：工程和技术（综合）

来源: Polska Akademia Nauk * Centrum Upowszechniania Nauki / Polish Academy of Sciences, Center for the Advancement of Science

【 摘 要 】

Seeding of cells on functional, biocompatible scaffolds is a crucial step in achievement the desired engineered tissue. In the present study, a pulsed laser modification onto inorganic substrate was made to promote endothelium cells migration and spread. Presented scaffolds were fabricated on carbon and titanium based coatings. Fabricated films provided very good mechanical properties together with a chemical stability preservation. The substrate modification consisted of grid-like template fabrication of micrometer size meshes. The microstructure analysis of laser traces revealed the grain size increase in the zone of laser beam interaction, which exerts an influence on a surface topography. Endothelium cells locomotion was observed within 10 day time period. As a result it was shown that the modified area enhanced cells adhesion with a preferred static behavior. The performed research work improved our understanding on the pulsed laser ablation process and template size influence on cells spatial arrangement. It constituted an important step towards fabrication of inorganic, biocompatible scaffolds for successful substrate endothelialization.

【 授权许可】

Unknown