Thin layer chromatography (TLC) technique in the investigation of artemisinin production in Artemisia annua L. medicinal plant hairy roots

Dynamic Chiropractic

关键词: Artemisia annua;    Agrobacterium rhizogenes;    hairy root;    thin layer chromatography (TLC) technique and artemisinin.;   

DOI  :  10.5897/JMPR11.846

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Artemisia annuaL. is an important medicinal plant, which is used for the treatment of malaria. Artemisinin is the active secondary metabolite in this plant and it is a sesquiterpen. This plant was studied for artemisinin production in hairy root induced byAgrobacterium rhizogenes. Polymerase chain reaction (PCR) forrolB gene confirmed the transformed hairy roots induced byA. rhizogenes. Then, thin layer chromatography (TLC) was employed for investigating the characteristics and quality of artemisinin in extracts of hairy roots and control roots ofA. annua. Result of TLC showed the presence of artemisinin in the induced hairy roots.

【 授权许可】

Unknown